CSB-E04486h人活化素A(ACV-A)ELISA Kit

1Human Activin A (ACV-A)ELISA KitCatalog No. CSB-E04486h(96T) This immunoassay kit allows for the in vitro quantitative determination of humanACV-A concentrations in serum, plasma. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.2PRINCIPLE OF THE ASSAYThe microtiter plate provided in this kit has been pre-coated with anantibody specific to ACV-A. Standards or samples are then addedto the appropriate microtiter plate wells with a HorseradishPeroxidase (HRP)-conjugated antibody preparation specific forACV-A and incubated. Then substrate solution A and B are addedto each well. Only those wells that contain ACV-A, HRP-conjugatedantibody will exhibit a change in color. The enzyme-substratereaction is terminated by the addition of a sulphuric acid solutionand the color change is measured spectrophotometrically at awavelength of 450 nm ± 2 nm. The concentration of ACV-A in thesamples is then determined by comparing the O.D. of the samplesto the standard curve.DETECTION RANGE66.6 pg/ml-2000 pg/ml. The standard curve concentrations used forthe ELISA’s were 2000 pg/ml, 1000 pg/ml, 500 pg/ml, 166.6pg/ml,66.6pg/ml.SPECIFICITYThis assay recognizes human ACV-A. No significantcross-reactivity or interference was observed.3SENSITIVITYThe minimum detectable dose of human ACV-A is typically lessthan 16.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) wasdefined as the lowest concentration that could be differentiatedfrom zero.MATERIALS PROVIDEDReagent QuantityAssay plate 1Standard(S1-S5) 5HRP-conjugate 1 x 6 mlWash Buffer1 x 15 ml(20×concentrate)Substrate A 1 x 7 mlSubstrate B 1 x 7 mlStop Solution 1 x 7 mlStandard S1 S2 S3 S4 S5Concentration(pg/ml)66.6 166.6 500 1000 20004STORAGE1. Unopened test kits should be stored at 2-8C upon receipt andthe microtiter plate should be kept in a sealed bag. The test kitmay be used throughout the expiration date of the kit, provided itis stored as prescribed above. Refer to the package label for theexpiration date.2. Opened test plate should be stored at 2-8C in the aluminum foilbag with desiccants to minimize exposure to damp air. The kitswill remain stable until the expiring date shown, provided it isstored as prescribed above.3. A microtiter plate reader with a bandwidth of 10 nm or less andan optical density range of 0-3 OD or greater at 450nmwavelength is acceptable for use in absorbance measurement.REAGENT PREPARATION1. Bring all reagents and plate to room temperature for at least 30minutes before use. Unused wells need store at 2-8°C andavoid sunlight.2. Wash Buffer If crystals have formed in the concentrate, warmto room temperature and mix gently until the crystals havecompletely dissolved. Dilute 15 ml of Wash Buffer Concentrateinto deionized or distilled water to prepare 300 ml of WashBuffer.5OTHER SUPPLIES REQUIRED Microplate reader capable of measuring absorbance at 450 nm,with the correction wavelength set at 540 nm or 570 nm. Pipettes and pipette tips. Deionized or distilled water. Squirt bottle, manifold dispenser, or automated microplatewasher. An incubator which can provide stable incubation conditions upto 37°C±0.5°C.SAMPLE COLLECTION AND STORAGE Serum Use a serum separator tube (SST) and allow samplesto clot for 30 minutes before centrifugation for 15 minutes at1000 g. Remove serum and assay immediately or aliquot andstore samples at -20°C. Centrifuge the sample again afterthawing before the assay. Avoid repeated freeze-thaw cycles. Plasma Collect plasma using citrate, EDTA, or heparin as ananticoagulant. Centrifuge for 15 minutes at 1000 g within 30minutes of collection. Assay immediately or aliquot and storesamples at -20°C. Centrifuge the sample again after thawingbefore the assay. Avoid repeated freeze-thaw cycles.Note: Grossly hemolyzed samples are not suitable for use in this assay.6ASSAY PROCEDUREBring all reagents and samples to room temperature before use. It is recommendedthat all samples, standards, and controls be assayed in duplicate. All the reagentsshould be added directly to the liquid level in the well. The pipette should avoidcontacting the inner wall of the well.1. Standard Reconstitute the Standards with 0.5 ml of ddH2O,respectively. Allow the standard to sit for a minimum of 15minutes with gentle agitation prior to use.2. Set a Blank well without any solution. Add 50μl of Standard orSample per well. Standard need test in duplicate.3. Add 50μl of HRP-conjugate to each well (not to Blank well).Mix well and then incubate for 1 hour at 37°C.4. Complete remove the liquid. Then fill each well with WashBuffer (about 200μl), stay for 10 seconds and Spinning. Repeatthe process for a total of three washes. Complete removal ofliquid at each step is essential to good performance. After thelast wash, remove any remaining Wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean papertowels.5. Add 50μl of Substrate A and 50μl of Substrate B to each well,mix well. Incubate for 15 minutes at 37°C. Keeping the plateaway from drafts and other temperature fluctuations in the dark.76. Add 50μl of Stop Solution to each well when the first four wellscontaining the highest concentration of standards developobvious blue color. If color change does not appear uniform,gently tap the plate to ensure thorough mixing.7. Determine the optical density of each well within 10 minutes,using a microplate reader set to 450 nm.CALCULATION OF RESULTSUsing the professional soft "Curve Exert 1.3" to make a standard curve isrecommended, which can be downloaded from our web.Average the duplicate readings for each standard, control, andsample and subtract the average zero standard optical density.Create a standard curve by reducing the data using computersoftware capable of generating a four parameter logistic (4-PL)curve-fit. As an alternative, construct a standard curve by plottingthe mean absorbance for each standard on the x-axis against theconcentration on the y-axis and draw a best fit curve through thepoints on the graph. The data may be linearized by plotting the logof the ACV-A concentrations versus the log of the O.D. and thebest fit line can be determined by regression analysis. Thisprocedure will produce an adequate but less precise fit of the data.If samples have been diluted, the concentration read from thestandard curve must be multiplied by the dilution factor.8LIMITATIONS OF THE PROCEDURE The kit should not be used beyond the expiration date on the kitlabel. Do not mix or substitute reagents with those from other lots orsources. If samples generate values higher than the highest standard,dilute the samples with the appropriate Diluent and repeat theassay. Any variation in operator, pipetting technique, washingtechnique, incubation time or temperature, and kit age cancause variation in binding. This assay is designed to eliminate interference by solublereceptors, binding proteins, and other factors present inbiological samples. Until all factors have been tested in theImmunoassay, the possibility of interference cannot beexcluded.TECHNICAL HINTS Centrifuge vials before opening to collect contents. When mixing or reconstituting protein solutions, always avoidfoaming.9 To avoid cross-contamination, change pipette tips betweenadditions of each standard level, between sample additions,and between reagent additions. Also, use separate reservoirsfor each reagent. When using an automated plate washer, adding a 30 secondsoak period following the addition of wash buffer, and/orrotating the plate 180 degrees between wash steps mayimprove assay precision. To ensure accurate results, proper adhesion of plate sealersduring incubation steps is necessary. Substrate Solution should remain colorless or light blue untiladded to the plate. Keep Substrate Solution protected from light.Substrate Solution should change from colorless or light blue togradations of blue. Stop Solution should be added to the plate in the same order asthe Substrate Solution. The color developed in the wells willturn from blue to yellow upon addition of the Stop Solution.Wells that are green in color indicate that the Stop Solution hasnot mixed thoroughly with the Substrate Solution.

该公司产品分类: 科研试剂

供应德国沃申道夫WACHENDORFF编码器

德国沃申道夫WACHENDORFF编码器     德国Wachendorff(沃申道夫品牌由Rolf Wachendorff(沃申道夫19781117日在德国的Geisenheim创建,至今已有20多年的发展进程。德国Wachendorff(沃申道夫自动化公司为客户提供坚固的工业元件及智能化系统,专用在高精度的测量位移、角度及方向的工业场合中。坚固的工业级Wachendorff(沃申道夫编码器的正常质保期为五年,远远高于欧洲大部分品牌的一年质保期的期限。     其他工业电子产品。     具体产品有:     Opus 系列  OPUS A1 , OPUS A2 , OPUS Thor ,OPUS 21     WDG  系列  WDG 58B , WDG SSI , WDG 40A , WDG 80H , WDG 58V , WDG 58K, WDG 115T , WDG 115M     WDG 58B-1500-ABN-G24-K3     WDG 40A-500-ABN-I24-K2     WDG 53S-5000-ABN-I24-K2     WDG 90B-1024-AB-H30-S2     WDG 115T-2048-AB-R30-S3     WDG 115M-1800-ABN-G24-S3     SZG 65-1250-N-UP-G24-05     WDG-DPB1B-0013-C100-0CC     WDG-SL00G-1213-C100-CRW     WDG-S100G-0013-B150-PRL     WDG-C8B1B-0013-C100-CRW     WDGHA1005      WDGHA1210

 

Wachendorff(沃申道夫公司的核心价值观一直是为控制、检测、优化工控系统设计生产销售设备。Wachendorff(沃申道夫以产品广泛应用于恶劣的环境而赢得美誉,同时公司产品也大量应用于便携式或可移动的操作平台。 

SMLmAC精密漏电流传感器:漏电测量、漏电保护、防触电、小电流隔离测量

SMLmAC精密漏电流传感器:漏电测量、漏电保护、防触电、小电流隔离测量SMLxxACE-Pn-On/Sn, 特点:南京三盟科技有限公司研制生产的漏电流传感器(小电流传感器)● 可测量漏电流、小电流(毫安级电流测量),量程20uA-1mA;至10000mA各档次均可选择● 快速响应,无击穿现象,初级与次级高度隔离● 精度高(0.2,0.5级),线性度好,属于精密型漏电流传感器● 电源:Vcc=5V-24V DC 单电源,或者AC110V,220V,380V等● 极低的功耗● 灵敏度高● 穿孔式测量,安装简单,使用方便● 穿孔直径10-300mm,可以直接穿铜排!● 性能如此,价格十分低廉,完善的用户服务!批量供货

图:Sn.jpg

关键字:漏电流传感器,小电流传感器,漏电流测量,漏电保护,电流传感器, 小电流隔离测量 

该公司产品分类: 电阻器

HS706C.T.P4S.DUL轴承FAG

 HS706C.T.P4S.DUL轴承 HS706C.T.P4S.DUL轴承价格 HS706C.T.P4S.DUL轴承尺寸 HS706C.T.P4S.DUL轴承报价 精密角接触球轴承样本图

订购原装FAG轴承请拨打021-3653 9288【FAG HS706C.T.P4S.DUL轴承】尺寸规格参数:轴承型号:HS706C.T.P4S.DUL轴承轴承系列:精密角接触球轴承轴承品牌:FAG轴承内径:6 外径:17 厚度:6 动载荷(Cr):1.56 静载荷(Cor):0.7 脂润滑限速:150000油润滑限速:220000净重kg:0.01 产品描述:钢球,15度,树脂保持架,P4级,开式,对装自由配组    上海东晟轴承有限公司提供 HS706C.T.P4S.DUL轴承的轴向压力、滚粒的数目、动态负荷、静态负荷、直径、替代型号等技术参数,我公司代理经营美国FAG轴承,瑞典SKF轴承,日本 NSK、NTN、IKO轴承,德国FAG、INA轴承、NADELLA轴承等名优进口轴承,如果您对 6 mm×17 mm×6 mm   FAG HS706C.T.P4S.DUL轴承有疑问,请联系我们获取更多HS706C.T.P4S.DUL轴承的最新信息。TEL:021-3653 9288   我公司专业现货供应精密角接触球轴承 HS706C.T.P4S.DUL轴承,我们为您推荐以下型号: B7004-E-T-P4S轴承  R18/16-8  23096BKD1  NN3026轴承  PHS3EC  HC7004-C-T-P4S轴承  7036CD/DT  W036  6320-2Z轴承  4T-HM911242/HM911210  6301-2RZ轴承  6016-RS1  2207K  NA4826轴承  SD544  FAG HS706C.T.P4S.DUL轴承   我公司还为您推荐FAGHS706C.T.P4S.DUL轴承广泛应用于: 齿轮泵, 手动泵, 钉跟机, 玻璃反应釜, 移印机, 食品药品包装机, 化纤机械, 塑料板材设备, 气流干燥设备, 等领域。EGBZ0812-E40轴承 http://www.fag.sh.cn/zhoucheng/EGBZ0812-E40.htmlEGBZ0814-E40轴承 http://www.fag.sh.cn/zhoucheng/EGBZ0814-E40.htmlC081008轴承 http://www.fag.sh.cn/zhoucheng/C081008.htmlBCE85轴承 http://www.fag.sh.cn/zhoucheng/BCE85.htmlK20X26X12轴承 http://www.fag.sh.cn/zhoucheng/K20X26X12.htmlL812147/L812111轴承 http://www.timken.sh.cn/TIMKEN/L812147_L812111.htmlL812148/L812111轴承 http://www.timken.sh.cn/TIMKEN/L812148_L812111.html29590/29520轴承 http://www.timken.sh.cn/TIMKEN/29590_29520.html29590/29522轴承 http://www.timken.sh.cn/TIMKEN/29590_29522.html395A/394轴承 http://www.timken.sh.cn/TIMKEN/395A_394.htmlEWP211轴承 http://www.nsk.sh.cn/bearings/EWP211.htmlEWP212轴承 http://www.nsk.sh.cn/bearings/EWP212.htmlEWP305轴承 http://www.nsk.sh.cn/bearings/EWP305.htmlAX4.5120155轴承 http://www.nadella.sh.cn/bearings/AX4.5120155.html

该公司产品分类: 轴承

KEM库仑法水分仪MKC-710系列

KEM库仑法水分仪MKC-710系列KEM库仑法水分仪MKC-710系列主要特点:MKC-710M:触摸屏和操作单元之间无线连接,同时控制四台自动滴定测量单元。通过使用无线适配器(蓝牙),操作单元和滴定装置可以不用电缆连接。在测量样品过程中有危害性气体时,可将滴定装置放置在排烟柜中,由无线传输的操作单元执行滴定。MKC-710M/MKC-710S:独创电解技术,最快2.6mg/min。可减少预滴定和测量的时间。KEM库仑法水分仪MKC-710系列技术参数:

形式

卡尔费休水分测定仪

仪器型号

MKC-710M

MKC-710S

MKC-710B

仪器组成

MCU-710M+MKC-710溶剂交换单元(选购)

MCU-710S+MKC-710溶剂交换单元(选购)

MKC-710溶剂交换单元(选购)

测量方法

卡尔费休库仑电量法

测量范围

水分含量: 10ug~300mgH2O(最大极限1000mg)溴值溴指数含量

测定池

隔膜式测量池或无隔膜式测量池

精确度

相对标准差:小于0.3%(n=10),依据标准测量方法和标准物质

显示分辨率

0.1μg

控制方法

定电流脉冲时间控制

终点检测

双铂检测电极交流极化法

终点判断方法

漂移稳定判断,或设定测量时间

试剂需求量

阳极液: 100mL(最多150mL),阴极液: 5mL

测量方法

120组测量方法

20组测量方法

按键操作

触摸屏

按键

显示

1) 8.4英寸彩色液晶屏,800x600点

1)白色LED液晶屏

2)英文/日文/中文/韩文/俄文/西语/德语/法语

2)英文/日文/中文/韩文/俄文/西语

3)四个通道同时显示(可同时连接电位滴定仪或容量法/库仑法水分仪)

3)一个通道显示

3)一个通道显示

计算

浓度计算,统计计算(平均值,标准差,相对标准差),自动输入空白值

数据储存

500组样品结果

100组样品结果

GLP认证

登记操作者/使用群组管理性能检查:提醒计划检查日期/记录检查结果试药寿命:提示试药更换日期和试药反应能力时间管理:显示操作时间

登记操作者/记录检查结果/药更换日期/试药反应能力/管理测量时间

外部输出

RS-232Cx4打印机,电子天平,数据软件(SOFT-CAP),水分蒸发器,多样品自动进样器

RS-232Cx2打印机,电子天平,数据软件(SOFT-CAP)

USBx1U盘,热敏打印机,A4打印机,键盘,条码机,脚踏开关,USB集线器

USBx1U盘,打印机,键盘,条码机,脚踏开关,USB集线器,安卓设备

SS-BUS x 1 : APB

LAN x 1 :电脑(PC)

扩充功能

测量单元:电位滴定仪(AT-710)容量水分仪(MKV-710)库仑水分仪MKC-710)最多四台测量单元

自动活塞滴定管:最多可控制2台滴定管驱动单元

水分蒸发器: ADP-611

使用环境

1)温度: 5~35°C2)相对湿度: 85%RH以下

电源

AC100~240V±10%,50Hz/60Hz

耗电量

主机:约30瓦打印机:约7瓦

主机:约20瓦打印机:约7瓦

尺寸

触摸屏: 225(W)x190(D)x42(H)mm

滴定单元: 141(W) x292(D) x244(H)mm(不包括管路)搅拌器: 107(W) x206(D) x340(H)mm(不包括溶剂交换单元)溶剂交换单元: 240(W) x140(D) x405(H)mm(不包括管路)打印机: 106(W) x180(D) x88(H)mm

重量

触摸屏:约1.5公斤

滴定单元:约3.0公斤搅拌器:约2.0公斤溶剂交换单元:约0.6公斤打印机:约0.4公斤

 

该公司产品分类: 电磁旋转粘度计 热分析相关仪器 石油产品专用仪器 食品饮料专用仪器 热流计、热流仪 导热仪、热导仪 糖度计、糖度仪 折光仪、折射计 密度计、密度仪 卡氏水分测定仪 自动电位滴定仪

CKB200系列CKB型单向楔块超越离合器

 

CKB型(B200型)单向楔块超越离合器

 

特性及应用

B200型号为无内环和轴承支撑的楔块式单向离合器,使用时需配合轴承安装以承受轴向和径向负载,使用者亦需要提拱润滑油脂供应及外部油封。

外环与机壁用键槽方式安装,轴直接安装在离合器内,所以用于离合器安装的轴在磨削后需要硬化处理到HRC58~62,轴的锥度每50mm不应超过0.01mm,适用于极限转速:1000~2400r/min公称转矩为60~1250N.m。常用于包装机械、冶金机械、矿山机械和石油机械、化工机械、水泥机械、电站等机械传动。

 

              

CKB无内环的单向楔块型超越离合器基本参数和主要尺寸        长度单位:mm

 

安装参考范例(Mounting e×ample)

新款HCH-3000E/E 回波-回波超声波测厚仪价目表

 新款HCH-3000E/E 回波-回波超声波测厚仪价目表

发射-回波模式:标准测量,用于普通的厚度测量。这种模式具有宽泛的测厚范围:0.8—500mm(根据具体探头)

回波-回波模式:能够穿透涂层直接对基体测厚,无需耗费时间和费用去除涂层后再测量;测厚范围:3.0—20mm

厚度显示分辨率:0.01mm;

探头零位:自动探头零位调整以满足众多探头在不同的测试条件下的佳参数设置,能够有效的避免由于探头的磨损或者温度变化等因素对测量结果产生的影响

自动灵敏度增益优化,可根据工作模式自动调节灵敏度,以得到更加精确的测量结果;

探头自动识别,可选多种原厂探头,每次开机自动识别探头;根据探头自动条调整灵敏度,自动探头零位调整;

小值扫描模式,每10次,快速测量并捕捉小值

上下限报警模式:可设置上下限报警阀值,当测量厚度超过阀值时,则给出报警信号;

差值模式:测量的厚度与设置的基值进行比较,并在屏幕上将差值显示出来;

数据分组化管理,一共分6组,每组都可存储100个数据。用户可根据自己的需要来选择组号和数据号作为目标存储地址或者查看测量数据。也可以删除某一个数据,或某一组数据,或者全部的数据。

打印输出,与PC通信,可将测量报告直接送至打印机输出也可传输至电脑上显示出来;

该公司产品分类: 湿海绵针孔检漏仪 里氏硬度计 黑白密度计 测振仪 个人剂量仪 紫外线照度计 埋地管道检测仪 观片灯 电火花检漏仪 涂层测厚仪 超声波测厚仪

RHB-32ATC

华科光学仪器有限公司是生产手持式折射仪的专业企业, 其产品广泛应用于饮料,酒类中糖度和酒精度的检测;临床医学中尿比重的测定;水产养殖中海水盐度测定以及工业用防冻液电池液浓度测定。使用方便,精确度高。产品远销欧洲,北美及东南亚。

B47C破碎机

    B47C破碎机   B47C破碎机/风镐  B47C气动破碎机

  B47C破碎机是以压缩空气为动力的破碎工具,能高效完成钢筋混凝土、岩石、沥青等破碎工作,寿命更长等特点,特别适宜对特大、特厚、特坚硬物体的破碎作业,是矿山、桥梁、道路、市政建设等基础工程施工的理想工具。
B47C破碎机技术规格:  
  重   量:  21.5kg 
  长   度:  550mm                       
  钎尾规格: 25×108 mm              
  气管内径: 19mm   
  耗 气 量: 21.5L/S                   
  冲击频率: 21.6Hz 
  钎具固定方式:开启式
组成与原理:
由配气机构、冲击机构和镐钎等组成,另一端通入气缸,各气孔的长度根据冲击锤的运动要求配置,以便轮流进气或排气,柱塞阀在螺旋弹簧作用下处于切断气路的常闭状态,使冲击锤在气缸内有规律地往复运动。冲击锤向前运动时,锤头打击钎尾箱.
联系人:王林
手  机:18764769169
 Q Q:  598298090
地  址:济宁高新区58号
该公司产品分类: 电机车 电机车配件 电泵风泵 凿岩机械

LXSZIC卡水表 立式水表 卡表 插卡水表 刷卡水表 阶梯水价水表

■功能概述:

●预付功能

预付水量:以水量冲入水表中,用水时从表中扣减水量,用完后关阀停水;

预付金额:以金额冲入表中,用水时参照价格文件扣件金额,用完后关阀停水;

●一卡通:

可实现水表、电表、气表等住宅计量仪表公用IC卡;

●余量报警:

剩余水量(金额)小于报警限值时,则报警关阀一次,提示购水,可插用户卡开阀;

●透支水量:

可设置水表透支水量,应急情况可使用该功能,下次充值后自动扣减;

●报警提示:

电池欠压、强磁干扰、阀故障、阀漏水等故障信息及时报警并显示;

●掉电关阀:

当电池欠压时,阀门会自动关闭,停止供水;

●囤水限量(额):

水表对用户购水量(金额)有限制,防止人为囤水;

●数据查询:

可随时查看水表本次购买量(费用)、剩余量(费用)、使用量(费用)等信息;

●数据冻结:

水表保存最近12个月的冻结数据,可带回系统做月结算及数据统计;

●数据安全:

采用逻辑加密卡,硬件与软件加密相结合,数据安全可靠;

●阀门维护:

阀门定时旋转,及时清掉阀门水垢,保证阀门灵活性;

●电池更换:

用户可在管理部门指导下,方便更换电池。河北恒亚科技有限公司http://www.hbhytech.com

该公司产品分类: 暖通阀 防污阀 排气阀 过滤器 软密封闸阀 水力控制阀 超声波热量表 超声波流量计 远传水表 智能卡式水表

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